Academic Writing

Discuss the use of synthetic biology and metabolic engineering to carryout chemical synthesis and reactions that would otherwise be difficult due to technical limitations and/or safety concerns Abstract  Write this section last.  It should have one sentence on each of the following: o What the aim was o What you did (method) o What you found o What it means in the big picture of things (topic) Introduction The introduction should use the following subsections:  A general introduction to what a protein is and what it is used for: o Focus on protein structure dictates function. o Discuss how protein networks are formed within cells to carryout metabolism and metabolic reactions  A general overview of recombinant technologies o What is possible in terms of synthesizing genes (DNA synthesis), expressing them in cells and then purifying products from these cells o What is possible in terms of modifying proteins: rational design and directed evolution: See here: [login to view URL] engineering-applications-on-industrially-important-enzymes-candida- methylica-fdh-as-a-case-s - just concentrate on the key highlights.  An overview of metabolic engineering: See here [login to view URL] and use the references within. Page 1 of 2  Specific topics: o Engineering Crops for Biofuel Production: see here for a general background [login to view URL] primed-for-making-biofuel/ you will go into more detail in the results section. o Metabolic engineering of Chinese hamster ovary cells: see [login to view URL] pharmaceuticals-06-00579 just use the high-level themes no specifics yet. Talk about what CHO cells are and why they are useful for expressing proteins. Methods As we discussed – just have a few lines and then use a flow diagram for what you On …[date] a search of .[databases]. using the search criteria …. This gave …. Journal articles  [number]… journal articles were retained because ….. the rest were rejected because ….   Discussion This is where I want you to go into detail about the two topics you introduced in the introduction. You should review some papers on each of the subjects – use the papers to assemble your reference list (i.e. direct your reading/ref list) and discuss  Some practical aspects of the systems – e.g. how plants are genetically modified to generate biofuels – specifics on the expression vectors, plant Page 2 of 2 strains etc… How CHO cell metabolism can be tuned to optimally express recombinant proteins.  See if you can find any new/innovative technologies in these areas to talk about.  The key think about the discussion is that you need to be critical in what you talk about Conclusions: You should try and bring together some sort of ending statement bringing together the technological innovations that have made it possible for us to modulate the expression and/or production of important proteins/products. References: You should have > 30 references by the end.